商品编号


AG-40T-0513-C400



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

400 ?g

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

Rabbit Erythrocytes



Crossreactivity

Multi-species



Formulation

Liquid.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The enzyme fractions supplied represent the full compliment of purified conjugation enzymes (E1, E2s, and E3s) that are found in mammalian Fraction II. Conjugation Fraction A contains predominantly E1 and E2 enzymes, while Conjugation Fraction B contains predominantly E3 and deubiquitinating enzymes. The enzymes have been tested and shown to work with typical [125I]-labeled substrate proteins such as lysozyme and β-lactoglobulin. These conjugation fractions contain deubiquitinating enzymes (DUBs) and ubiquitin C-terminal hydrolases (UCHs). The addition of ubiquitin aldehyde is recommended for the inhibition of these activities to improve overall conjugate yield. The supplied fractions do not contain 20S or 26S proteasome degradation activity. If the substrate being conjugated requires E2 or E3 enzymes not found in Fraction II, the reaction can be supplemented with enzymes from Fraction I. Includes complimentary fractions for 2-5 conjugation reactions depending on conditions. Conjugation kit with solutions and protocol is also available in a kit format.