商品编号


GWB-7A9F7E



品牌


GenWay



公司


GenWay



公司分类


Secondary Antibodies



Size

0.5 mg

商品信息

Description:
Short Description:
MOUSE ANTI RAT IgG2b HEAVY CHAIN
Additional Information:




Name

Rat IgG2b Heavy Chain Antibody



Related Product Names

MOUSE ANTI RAT IgG2b HEAVY CHAIN IgG2bIgh-3



Gene Symbol

Igh-1a



Gene id

299352



Alias Symbols

Igh-1a



Protein Name

Ig gamma-2B chain C region



Description of Target

MOUSE ANTI RAT IgG2b HEAVY CHAIN



Swissprot ID

P20761



Species Reactivity

Rat



Clone

MARG2b-3



Host

Mouse



Isotype

IgG1



Immunogen

Rat IgG2b



Homology

Rat



Product Format

Purified IgG - liquid



Specificity

IgG2b



Concentration

1mg/ml



Applications

E



Application Info

ELISA
:
This antibody may be used as a coating antibody in a sandwich
ELISA
in combination with clone MARK-1/MARL-15 (GWB-9BA2B896P) as detection reagent and purified rat IgG2b (GWB-BB9356).



Clonality

Monoclonal



Format

Solution: Phosphate buffered saline, Stabalizer: 0.1%, Sodium Azide, Form: Purified IgG - liquid



St
ABI
lity

18 months from date of despatch.



Reconstitution and Storage

Store at +4
o
C or at -20
o
C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.



Reactivity

Rat



Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate



Protocol Information

Citation:
1: Yang CP, Shittu E, Bell EB. Specific B cell tolerance is induced bycyclosporin A plus donor-specific blood transfusion pretreatment: prolongedsurvival of MHC class I disparate cardiac allografts. J Immunol. 2000 Mar1;164(5):2427-32. PubMed PMID: 10679079.
Species:
Rat
Experiment Name:
1. Cell separation2. Alloantibody determination by flow cytometry3. Cytotoxic Ab determination
Experiment Background:
1. Using a published protocol for prolonging the survival of kidney allografts in the high responder RT1u strain of rat, Chun-Ping Yang, Emma Shittu, and Eric B. Bell determined whether the same preoperative treatment was effective in enhancing survival of cardiac allografts.2. An examination of Ab production in CD4 T cell-injected nude rats indicated that the onset of rejection in the control group corresponded with production of cytotoxic Ab. 3. Anti-MHC class I Ab production in R8 heart-grafted, CD4 T cell-reconstituted RT1u nude rats
Experimental Steps:
1. Cell separationThoracic duct lymphocytes were depleted of B cells and CD8 T cells using a mixture of mouse anti-rat mAbs grown in house as ascites or purchased from (Kidlington, Oxford, U.K.): OX12 (anti-Igk), OX6 (anti-MHC class II), and OX8 (anti-CD8). Stained lymphocytes were removed by two or three rounds of magnetic adherence using anti-mouse Ig-conjugated immunomagnetic particles. The resulting population of cells was 95–98% CD41. B cells were obtained from thoracic duct lymph of athymic nude rats; 80–85% of cells were Ig+ and void of functioning T cells.2. Alloantibody determination by flow cytometrySera from RT1u animals were serially diluted and mixed with RBC from R8 rats to detect anti-MHC class I (Aa) alloantibody. After incubation, the red cells were washed and stained with FITC-sheep anti-rat IgG or with mouse mAb against rat IgG1 (MARG1-2), IgG2a (MARG2a-1), and IgG2b (MARG2b-3) , followed by FITC-F(ab’)2 anti-mouse Ig (Dako, High Wycombe, U.K.) absorbed against solid phase rat Ig. The titer for each sample was determined as the reciprocal dilution (log3) at which the percentage of positively stained cells was.>5% above that of a normal rat serum control.3. Cytotoxic Ab determinationThe procedure was adapted from that of Bradley et al.. Briefly, 50 ?l of serially diluted test sera, in duplicate, was added to 96-well roundbottom microtiter plates (Alpha Laboratories, Eastleigh, U.K.), and 50 ?l of 51Cr-labeled Con A-stimulated blast cells at 106 per ml in RPMI 1640 plus 10 mM HEPES with 5% FCS were added to each well. Plates were incubated for 30 min at 37°C, 100 ml of 1/25 diluted baby rabbit complement was added, and the plates were incubated for 1 h at 37°C. Maximum release was obtained by the addition of 100 ml HCl in place of complement. The plates were centrifuged, and 100-ml aliquots of supernatant containing released 51Cr were removed and counted. Specific release was calculated by the formula: 100 x [(experimental release - spontaneous release)/(maximum release - spontaneous release)].
Number Of Protocols:
3



Lead Time

Domestic: within 2-3 weeks delivery?International: 2-3 weeks



Intended Use

Research Use Only



Key Reference

1. LeRoy, D. et al. (1999) Monoclonal antibodies to murine lipopolysaccharide (LPS)-binding protein (LBP) protect mice from lethal endotoxemia by blocking either the binding of LPS to LBP or the presentation of LPS/LBP complexes to CD14. J. Immunol. 162: 7454 - 7460.



::

Preparation:
Purified IgG prepared by affinity chromatography
Preservative St
ABI
lisers:
0.09% - Sodium Azide



::

Approx Protein Conc:
IgG concentration 1 mg/ml
Buffer Solutions:
Phosphate buffered saline