商品编号


AG-40T-0459ML-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin (Accession Nr. P0CG47) conjugated to AML.



Crossreactivity

Human



Label/Conjugates

AML



Formulation

Liquid. In HEPES.



Other Product Data


Use:
Substrate for deubiquitinating enzymes (DUBs) based on the C-terminal derivative of ubiquitin with Aminoluciferin (AML). Rather than fluorescence as the indicator of DUB activity, DUB liberated luciferin is processed by luciferase to give a luminescence signal. Produces a stronger signal and has an excellent signal to noise ratio over tr
ADI
tional fluorophores. This makes it poss
IBL
e to rapidly assess the activity of DUBs that poorly utilize Ubiquitin-AMC while using much lower levels of the DUBs themselves. Ideal for use as a ubiquitin-specific isopeptidase enzyme substrate. Isopeptidase activity liberates luciferin from this protein. ATP and luciferase are then added to produce a luminescent signal proportional to ubiquitin-specific isopeptidase activity. Optimal luminescence at pH 7.5 can be monitored using all wavelengths with a 500 ms integration time. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-1?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.