商品编号


AG-40T-0358-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Signal Transducing Adapter Molecule 1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Full-length human STAM-1 (Accession Nr. Q92783).



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 2mM DTT, 2mM EDTA .



Other Product Data


Use:
Has no intrinsic deubiquitinase activity but is useful in stimulating the
in vitro
activity of the JAMM-class deubiquitinase AMSH. Reaction conditions for stimulation of AMSH will need to be optimized for each specific application. Binds AMSH protein with Kd = ~7?M. In
in vitro
assays containing 200nM AMSH, a ~10-fold increase in the rate of hydrolysis of K63-linked diubiquitin FRET substrate was achieved upon the addition of STAM. Half-maximal increase occurred at 0.4?M STAM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Signal Transducing Adaptor Molecule-1 (STAM-1) is a member the STAM family of adaptor molecules. This ubiquitously expressed protein is 540 amino acids (aa) in length with a predicted molecular weight of 59.18 kDa. Human STAM-1 shares 91% and 92% aa sequence identity with the rat and mouse orthologs, respectively. It contains multiple domains including a VHS domain (aa 16-143) and a ubiquitin-interacting motif (aa 171-190), both of which bind ubiquitinated proteins, a SH3 domain (aa 210-269) that associates with the zinc metalloprotease STAMBP and an ITAM domain (aa 370-387), which contains a Hrs-binding site and interacts with Janus kinases (Jaks). STAM-1 is involved in downstream signaling stimulated by cytokines and growth factors. It is tyrosine phosphorylated by Jaks in response to a variety of cytokines including IL-2, IL-3, IL-4, IL-7, GM-CSF, EGF and PDGF, and has been shown to induce DNA synthesis and MYC expression. Additionally, STAM-1 has been shown to regulate CXCR4 signaling and appears to be important for T cell development and survival. Besides its role in signal transduction, STAM-1 may also be important for the regulation of endocytic membrane trafficking. It associates with Hrs to form the ESCRT-0 complex, which binds to ubiquitinated membrane proteins on early endosomes and directs them to the ESCRT-1 complex for lysosomal degradation. STAM-1 has also been shown to interact with coat protein II complexes and function in trafficking vesicles from the endoplasmic reticulum to the golgi apparatus.