商品编号


AG-40T-0010-C020



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

20 ?g


商品信息


More Information



Product Details



Synonyms

Poly(ADP-ribose) Polymerase 1; ADP-ribosyltransferase Diphtheria Toxin-like 1; NAD(+) ADP-ribosyltransferase 1



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human PARP-1 [ARTD1] (aa 656-1014) is fused to a MYC and His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

~50ng are required for poly(ADP-ribose) formation.



Purity

≥90% (SDS-PAGE)



Concentration

Lot dependent (0.2-1.5mg/ml)



Formulation

Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol.



Other Product Data

UniProt ID P09874:?PARP-1 (human)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





PARP-1 (ARTD1) is involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway le
ADI
ng to the reparation of DNA strand breaks. PARP-1 positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. It forms a complex with EEF1A1 and TXK that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-γ to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.


Product References

The enzymatic and DNA binding activity of PARP-1 are not required for NF-kappa B coactivator function: P.O. Hassa, et al.; J. Biol. Chem.
276,
45588 (2001)