商品编号


AG-40T-0093-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Nicotinamide Mononucleotide Adenylyltransferase 3; NMN Adenylyltransferase 3; NaMN Adenylyltransferase 3; EC 2.7.7.18; EC 2.7.7.1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human full-length NMNAT3 (aa 1-252) is fused at the N-terminus to a His-tag.



Crossreactivity

Human



Application

Well suited for the synthesis of NAD analogs due to lower substrate selectivity compared to NMNAT1 (human) (rec.) (His) (highly active). For NAD synthesis use NMNAT1 (human) (rec.) (His) (highly active) (AG-40T-0092).



BIOLOG
ical Activity

≥2U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1?mol of NAD
+
per min.



MW

~27kDa (monomer)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

N/A



Concentration

1mg/ml



Formulation

Liquid. In 50mM TRIS-HCl, pH 8.0, containing 300mM sodium chloride, 1mM DTT and 10% glycerol.



Other Product Data

UniProt link Q96T66: NMNAT3 (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


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Product Specification Sheet



Datasheet


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Mitochondrial NMNAT isoform. Catalyzes the formation of NAD
+
from nicotinamide mononucleotide (NMN) and ATP. Can also use the deamidated form of nicotinic acid mononucleotide (NAMN) as substrate with the same efficiency. Can use tiazofurin monophosphate as substrate. Can also use GTP and ITP as nucleotide donors. Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD
+
. For the pyrophosphorolytic activity, mitochondrial NMNAT isoform can use NAD(
+
), NADH, NAAD, nicotinic acid adenine dinucleotide phosphate (NHD), nicotinamide guanine dinucleotide (NGD) as substrates. Fails to cleave phosphorylated dinucleotides NADP
+
, NADPH and NAADP
+
. Protects against axonal degeneration following injury.


Product References

Structural characterization of a human cytosolic NMN/NaMN adenylyltransferase and implication in human NAD biosynthesis: X. Zhang, et al.; J. Biol. Chem.
278,
13503 (2003)

Subcellular compartmentation and differential catalytic properties of the three human nicotinamide mononucleotide adenylyltransferase isoforms: F. Berger, et al.; J. Biol. Chem.
280,
36334 (2005)

The NMN/NaMN adenylyltransferase (NMNAT) protein family: C. Lau, et al.; Front. Biosci.
14,
410 (2009)

Isoform-specific targeting and interaction domains in human nicotinamide mononucleotide adenylyltransferases: C. Lau, et al.; J. Biol. Chem.
285,
18868 (2010)