商品编号


AG-40T-0302-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NEDD8 vinyl sulfone (Accession Nr. Q15843) fused to a FLAG-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM Hepes pH 6.5, 150mM NaCl, 150mM sodium acetate, 10% glycerol.



Other Product Data


Use:
Potent and irrevers
IBL
e inhibitor of NEDD8-specific isopeptidases (like NEDP1 or SENP8). Useful for inhibiting the hydrolysis of poly-NEDD8 chains on substrate proteins
in vitro
and thus enhances poly-NEDD8 chain accumulation. The FLAG-epitope allows for the sensitive identification or purification of NEDD8 deconjugating activities since it is specifically recognized by anti-FLAG antibodies M1, M2 or M5. Add directly to
in vitro
assay from the stock solution. Depending on conditions, typical concentrations to fully inhibit SENPs
in vitro
are 1-5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Rub1, is a 6-8 kDa member of the ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide. Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits. Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes. Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates. For example, transfer of NEDD8 to the cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of ubiquitin ligases (E3). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival. Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies.