商品编号


AG-40T-0299-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

NEDD8-activating Enzyme E1 C; Ubiquitin-activating Enzyme E1 C; UBE1C; APPBP1/UBA3



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NEDD8 E1 (Accession Nr. APP-BP1 | UBA3).



Crossreactivity

Human



Formulation

Liquid. In HEPES and NaCl.



Other Product Data


Use:
Member of the NEDD8-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a NEDD8-conjugating (E2) enzyme and a NEDD8 ligase (E3) to conjugate NEDD8 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 50-200nM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





NEDD8 Activating Enzyme 1 (APPBP1/UBA3) is a heterodimeric NEDD8-activating (E1) enzyme with a predicted molecular weight of 112 kDa. It is respons
IBL
e for the first step in the conjugation of NEDD8 to protein substrates. The NEDD8 activating enzyme heterodimer is composed of a regulatory subunit, APPBP1 and a catalytic subunit, ubiquitin-like modifier activating enzyme 3 (UBA3). Human APPBP1 is a 534 amino acid (aa) protein with a predicted molecular weight of 60 kDa that is expressed ubiquitously in fetal tissues and in the adult brain. APPBP1 is required for UBA3 neddylation activity, regulates enzyme specificity and is expressed as two isoforms, the full length protein and a second isoform with an alternate N-terminal, aa 1-17 sequence. APPBP1 has been shown to drive cell cycle progression and its expression is increased in the hippocampus of Alzheimer's disease brains. Human UBA3 is a 463 aa protein with a predicted molecular weight of 52 kDa. It is ubiquitously expressed and shares high aa sequence identity with the C-terminal domain of human UBE1. UBA3 contains an ATP-binding domain and an active site cysteine residue, Cys237 in humans, which are both common to E1 enzymes. Like APPBP1, two isoforms of UBA3 have been identified in humans, the full length protein and a truncated isoform, which lacks aa 8-21. UBA3 is required for cell cycle progression and has been shown to downregulate steroid receptor activation. Neddylation and its associated enzymes have been implicated in the progression of Alzheimer's disease, via neddylation of APP, and cancer via post-translational modification of oncogenes.