商品编号


SBB-PS0003-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NEDD8 (aa1-76) (Accession Nr. Q15843) conjugated at the C-terminus to a quenched Rhodamine 110 dye.



Crossreactivity

Human



Application

Protein-based substrate. Typical working range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.



Label/Conjugates

Rhodamine



MW

~9kDa



Purity

≥97% (LCMS)



Concentration

Lot dependent.



Accession Number

Q15843



Formulation

Liquid. In 50mM MES pH 6.0, 150mM sodium chloride.



Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by South Bay Bio.



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-80°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8) is a ubiquitin like protein that plays an important role in regulating development and the cell cycle. NEDD8 is conjugated to a target protein via a signaling cascade similar to ubiquitin: a NEDD8 specific E1 activating enzyme (APPBP1/UBA3) adenylates the C-terminus of NEDD8 which is than subsequently passed to an E2 conjugating enzyme (UBE2M or UBE2F) that facilitates covalent attachment of NEDD8 to a cullin subunit of an SCF E3 ubiquitin ligase. NEDD8-Rhodamine 110 can be used as a substrate for enzymes exhibiting deNEDDylating activity, e.g UCH-L3, UCH-L1, COP9 Signalosome and NEDP1.