商品编号


AG-40T-0293F-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NEDD8 (Accession Nr. Q15843) conjugated to FITC.



Crossreactivity

Human



Label/Conjugates

FITC



Formulation

Liquid. In 20mM HEPES pH 8.0.



Other Product Data


Use:
Modified with fluorescein via primary amine coupling is ideal as an alternative to r
ADI
olabeled NEDD8. This results in multiple fluoresceinated NEDD8 species with modified lysines as well as the N-terminus. Fluorescein-NEDD8 gives a strong signal in the range of 0.1-1μM, depending on exact experimental conditions. Optimal fluorescence at pH 8.0 is monitored using Ex 494nM and Em 520nM wavelengths, respectively.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Rub1, is a 6-8 kDa member of the ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide. Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits. Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes. Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates. For example, transfer of NEDD8 to the cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of ubiquitin ligases (E3). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival. Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies.