商品编号


AG-40T-0293ML-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8; Neddylin; Rub1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human NEDD8 (Accession Nr. Q15843) conjugated to AML.



Crossreactivity

Human



Label/Conjugates

AML



Formulation

Liquid. In 50mM HEPES pH 7.5, 100mM NaCl.



Other Product Data


Use:
Substrate for deconjugating enzymes (NEDPs), based on the C-terminal derivative of NEDD8 with aminoluciferin (AML). Rather than fluorescence as the indicator of NEDP activity, NEDP liberated luciferin is processed by luciferase to give a luminescence signal. NEDD8-AML not only produces a stronger signal, but also has an excellent signal to noise ratio over tr
ADI
tional fluorophores. This makes it poss
IBL
e to rapidly assess the activity of NEDPs that poorly utilize NEDD8-AMC while using much lower levels of the NEDPs themselves. Can be monitored using all wavelengths with a 500 ms integration time. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-1?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8), also known as Rub1, is a 6-8 kDa member of the ubiquitin family of proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. Pro-NEDD8 contains one ubiquitin-like domain (aa 1-65) and a 4-5 aa C-terminal propeptide. Cleavage of the propeptide exposes a C-terminal glycine residue that is used to generate a glycine-lysine intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from mouse, rat and canine. Human NEDD8 is activated by a distinct NEDD8-activating (E1) enzyme, a heterodimeric complex composed of APPBP1 and UBA3 subunits. Activated NEDD8 is subsequently transferred to the UBE2M/Ubc12 or UBE2F NEDD8-conjugating (E2) enzymes. Through a process termed neddylation, the ROC1/Rbx1 RING Finger E3 ligase transfers NEDD8 to specific substrates. For example, transfer of NEDD8 to the cullin scaffold activates members of the Cullin-RING Ligase (CRL) family of ubiquitin ligases (E3). CRL-induced ubiquitination is known to regulate proteins that are important for cell cycle progression and cell survival. Physiologically, NEDD8 plays a critical regulatory role in cell proliferation and dysregulation of the NEDD8 pathway has been associated with several cancer pathologies.