商品编号


AG-40T-0290-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

E3 Ubiquitin-protein Ligase Mdm2; Double Minute 2 Protein; HDM2



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human MDM2 (Accession Nr. Q00987-11) fused to a N-terminal GST-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, Glycerol and TCEP.



Other Product Data


Use:
RING finger ubiquitin ligase (E3) that functions downstream of a ubiquitin-activating (E1) enzyme and a ubiquitin-conjugating (E2) enzyme to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.5-4?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Double Minute 2 Protein (Mdm2, also known as Hdm2) is a RING-finger ubiquitin E3 ligase that acts as a major regulator of the tumor suppressor p53. Mdm2 inhibits p53-mediated cell cycle arrest and Cancer by binding its transcriptional activation domain. The E3 ligase activity is confined to the C-terminal domain of Mdm2 and is respons
IBL
e for the ubiquitination and subsequent proteasomal degradation of p53. Although the isolated RING domain is capable of p53 ubiquitination, other regions of the protein including a central acidic domain are also crucial for full E3 ligase activity. Mdm2 also regulates its own intracellular levels by auto-ubiquitination, and can be SUMOylated which decreases autoubiquitination activity but increases activity toward p53. Mdm2 also affects the cell cycle and Cancer through interactions with other proteins such as retinoblastoma1 (pRB) and ribosomal protein L5.