商品编号


AG-40T-0352B-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

SPF Chicken Eggs



Sequence

Lysozyme conjugated to biotin.



Crossreactivity

Chicken



Label/Conjugates

Biotin



Formulation

Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.



Other Product Data


Use:
Typical concentration to support conjugation
in vitro
is 5-20μM (70-280μg/ml) depending on conditions.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Lysozyme has been used extensively as a substrate to study the ubiquitin-dependent proteolytic pathway
in vitro
. This protein is an N-end rule substrate, since the recognition of protein requires the binding of an E3 ligase to the N-terminal lysine residue. Ubiquitin-lysozyme conjugates can be formed in the presence of ATP, ubiquitin and the appropriate conjugation enzyme source. This substrate protein can be used as a positive control with crude fractions and conjugation kits. Additionally, this lysozyme is labeled with biotin for the sensitive detection with avidin affinity reagents, but has all available lysines present for the efficient conjugation to ubiquitin.