商品编号


AG-40T-0285MC-C025



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; Microtubule-associated protein 1 light chain 3 α; Apg8; APG8a; Apg8p3; MLP3A; MAP1LC3A



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human LC3 (Accession Nr. NP_115903) fused to a N-terminal HA-tag and conjugated to AMC.



Crossreactivity

Human



Label/Conjugates

AMC



Formulation

Liquid. In 50mM Hepes pH 6.5, 100mM NaCl, 10 % glycerol.



Other Product Data


Use:
Fluorogenic substrate for Apg8-specific C-terminal hydrolases such as Apg4b, based on the C-terminal derivatization with 7-amido-4-methylcoumarin (AMC). ATG8E-AMC is useful for studying such enzyme activities when detection sensitivity or continuous monitoring of activity is essential. Substrate concentrations for assay range from 0.1-1μM depending onconditions. Release of AMC fluorescence can be monitored using Ex 380nM and Em 460nM wavelengths, respectively.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of autophagy-related 8 (Atg8) proteins. The LC3 subfamily also includes LC3B and LC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins. However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation. Recruitment of LC3 to the phagophore may promote membrane elongation. It may also be involved in cargo recruitment to autophagosomes. LC3 is often used as a
Marker
of autophagy.