商品编号


AG-40T-0286B-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Microtubule-associated proteins 1A/1B light chain 3A; Autophagy-related protein LC3 A; Autophagy-related ubiquitin-like modifier LC3 A; Microtubule-associated protein 1 light chain 3 α; Apg8; APG8a; Apg8p3; MLP3A; MAP1LC3A



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human LC3 (Accession Nr. NP_115903) fused to a N-terminal His-tag and conjugated to biotin.



Crossreactivity

Human



Label/Conjugates

Biotin



Formulation

Liquid. In 50mM Hepes pH 7.5, 100mM NaCl.



Other Product Data


Use:
Modified with biotin via primary amine coupling resulting in modification of lysine residues as well as the N-terminus. Biotinylated LC3 can be detected using avidin-linked reagents for higher efficiency and detection sensitivity than with antibodies. Can be conjugated to substrate proteins via the subsequent actions of a Apg-activating (E1) enzyme, a Apg-conjugating (E2) enzyme, and a Apg ligase (E3). It is ideal for the visualization or quantification of thioester formation with avidin-linked reagents. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 1-5?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of autophagy-related 8 (Atg8) proteins. The LC3 subfamily also includes LC3B and LC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins. However, LC3 has gained attention for MAP1-independent functions in autophagy. LC3 utilizes a ubiquitin-like conjugation system that includes E1-, E2-, and E3-like enzymes to covalently attach phosphatidylethanolamine (PE) to its C-terminus, incorporating it into the phagophore membrane during the early stages of autophagasome formation. Recruitment of LC3 to the phagophore may promote membrane elongation. It may also be involved in cargo recruitment to autophagosomes. LC3 is often used as a
Marker
of autophagy.