商品编号


AG-40B-0031-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Lymphocyte Activation Gene-3; FDC Protein; CD223



Product Type

Protein



Properties



Source/Host

CHO cells



Sequence


The sequence coding for the 4 extracellular Ig-like domains of human LAG-3 (D1-D4) is fused to the Fc portion of human IgG1.




Crossreactivity

Human
Monkey
Mouse



Specificity


Binds to human, mouse and monkey MHC class II.




BIOLOG
ical Activity


Inhibits binding of MAb to LAG-3 (human) to LAG-3. Induces maturation of human dendritic cells.




MW

~80kDa (SDS-PAGE)



Purity

≥99% (SDS-PAGE)



Endotoxin Content

<0.001eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.001eu>



Concentration

0.5 mg/ml



Formulation

Liquid. In PBS containing 5mM arginine.



Other Product Data


Application: ?
Flow Cytometry: Detection of MHC class II molecules in combination with fluorescently labeled antibodies to IgG1. Product contains arginine for optimized protein st
ABI
lity.?
For Labeling: Use
AG-40B-0031RF
(Arginine-Free) - Please Inquire!
Uni-Prot link P18627: LAG-3 (human)




Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.
Contains arginine for optimized protein st
ABI
lity.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.


Product References

CD4/major histocompatibility complex class II interaction analyzed with CD4- and lymphocyte activation gene-3 (LAG-3)-Ig fusion proteins: B. Huard, et al.; Eur. J. Immunol.
25,
2718 (1995)

T cell major histocompatibility complex class II molecules down-regulate CD4+ T cell clone responses following LAG-3 binding: B. Huard, et al.; Eur. J. Immunol.
26,
1180 (1996)

Maturation and activation of dendritic cells induced by lymphocyte activation gene-3 (CD223): S. Andreae, et al.; J. Immunol.
168,
3874 (2002)

LAG-3 (CD223) reduces macrophage and dendritic cell differentiation from monocyte precursors: S. Buisson & F. Triebel; Immunology
114,
369 (2005)

A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett.
235,
147 (2006)

A soluble form of lymphocyte activation gene-3 (IMP321) induces activation of a large range of human effector cytotoxic cells: C. Brignone, et al.; J. Immunol.
179,
4202 (2007)