商品编号


CHI-ZHF-21002S-3010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

3 x 10 ?g

商品信息


More Information



Product Details



Synonyms

IL-2 Superkine (human):Fc (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; Aldesleukin; Super-2



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

The extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) is fused at the C-terminus to the Fc portion of human IgG2.



Crossreactivity

Human



Application

Triggers far greater antitumor responses than native IL-2
in vivo
but with lower toxicity.



Specificity

Binds to human and mouse IL-2R.



MW

42kDa



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" protein="" (lal="" test;="" lonza).="">0.01eu>



Concentration

0.1mg/ml after reconstitution.



Reconstitution

Reconstitute with 100μl sterile water.



Formulation

Lyophilized. Contains PBS.



Other Product Data

Uniprot link P60568 :?IL-2 (human)
AfCS Signaling Gateway link A006222: IL-2 (mouse)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After reconstitution, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.
IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors. IL-2 Superkine (Fc) is an artificial variant of IL-2 containing mutations at positions L80F / R81D / L85V / I 86V / I92F. These mutations are located in the molecule's core that acts to st
ABI
lize the structure and to give it a receptor-binding conformation mimicking native IL-2 bound to CD25. These mutations effectively eliminate the functional requirement of IL-2 for CD25 expression and elicit proliferation of T cells. Compared to IL-2, the IL-2 superkine induces superior expansion of cytotoxic T cells, le
ADI
ng to improved antitumour responses in vivo, and elicits proportionally less toxicity by lowering the expansion of Tregulatory cells and reducing pulmonary oedema.


Product References

Exploiting a natural conformational switch to engineer an interleukin-2 'superkine': AM. Levin, et al.; Nature
484,
529 (2012)