商品编号


CHI-MF-12010-C010



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

10 ?g

商品信息


More Information



Product Details



Synonyms

Interleukin-10; Cytokine Sythesis Inhibitory Factor; CSIF



Product Type

Protein



Properties



Source/Host

CHO cells



Sequence

The extracellular domain of mouse IL-10 (aa 1-178) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.



Crossreactivity

Mouse



BIOLOG
ical Activity

Shows the
BIOLOG
ical function of the IL-10 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.



Purity

≥98% (SDS-PAGE)



Endotoxin Content

<0.06eu mu;g="" protein="" (lal="" test;="" lonza).="">0.06eu>



Reconstitution

Reconstitute at 100μg/ml in sterile PBS.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
NCBI reference AAI37845.1: IL-10 (mouse)



Declaration

Manufactured by Chimerigen.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Interleukin-10 (IL-10) is a cytokine produced by activated Th2 cells, B cells, keratinocytes and monocytes/macrophages.
In vitro
murine and human IL-10 inhibits cytokine synthesis by Th1 cells, natural killer cells and monocytes/macrophages. Several studies have suggested the potential application of IL-10 as an anti-inflammatory agent in the treatment of septic shock and as an immunosuppressive agent in certain T cell mediated autoimmune diseases.


Product References

Admi
NIST
ration of noncytolytic IL-10/Fc in murine models of lipopolysaccharide-induced septic shock and allogeneic islet transplantation: X.X. Zheng, et al.; J. Immunol.
154,
5590 (1995)

Recombinant IL-10 and IL-10/Fc treatment down-regulate egg antigen- specific delayed hypersensitivity reactions and egg granuloma formation in schistosomiasis: P.O. Flores-Villanueva, et al.; J. Immunol.
156,
3315 (1996)

A noncytolytic IL-10/Fc fusion protein prevents diabetes, blocks autoimmunity, and promotes suppressor phenomena in NOD mice: X.X. Zheng, et al.; J. Immunol.
158,
4507 (1997)

IL-10/Fc inhibits macrophage function and prolongs pancreatic islet xenograft survival: X. Feng, et al.; Transplantation
68,
1775 (1999)

Critical role of Kupffer cell-derived IL-10 for host defense in septic peritonitis: K. Emmanuilidis, et al.; J. Immunol.
167,
3919 (2001)