商品编号


AG-40A-0030-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g


商品信息


More Information



Product Details



Synonyms

Indoleamine 2,3-dioxygenase; INDO



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Mouse IDO (aa 1-407) is fused at the C-terminus to a His-tag.



Crossreactivity

Mouse



BIOLOG
ical Activity

Specific Activity: >100’000U/mg protein with L-tryptophan as substrate and after addition of 7μM hemin for protein induction. One unit is defined as the amount of enzyme that produces 1nmol of kynurenine per hour.



MW

~40kDa (SDS-PAGE)



Purity

≥90% (SDS-PAGE)



Endotoxin Content

<1eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">1eu>



Concentration

1mg/ml



Formulation

Liquid. 0.2μm-filtered solution in 50mM TRIS-Cl, pH 7.4, containing 1mM EDTA.



Other Product Data

UniProt link P28776: Indoleamine 2,3-dioxygenase (mouse)
Note:
Heme content was not determined for the end product.



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-γ induce the expression of IDO in several tissues. IDO-dependent suppression of T cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.