商品编号


AG-40B-0161-C050



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

50 ?g



商品信息


More Information



Product Details



Synonyms

Indoleamine 2,3-dioxygenase; INDO; IDO1; IDO-1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human IDO (aa 1-403) is fused at the N-terminus to a His-tag.



Crossreactivity

Human



BIOLOG
ical Activity

Specific Activity: >100’000U/mg protein with L-tryptophan as substrate (activity assay with catalase). One unit is defined as the amount of enzyme that produces 1nmol of N-formylkynurenine (NFK) per hour. For more information see Activity Test Protocol.



MW

~47kDa (SDS-PAGE)



Purity

≥90% (SDS-PAGE)



Endotoxin Content

<0.1eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.1eu>



Concentration

Lot dependent



Formulation

Liquid. 0.2μm-filtered solution in 10mM Tris, pH 7.5, containing 100mM NaCl and 20% glycerol.



Other Product Data

UniProt link P14902: Indoleamine 2,3-dioxygenase (human)



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

After opening, prepare aliquots and store at -80°C.
Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



Protocols


Download PDF



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





IDO is a heme enzyme that catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway, causing depletion of tryptophan which can lead to halted growth of microbes as well as T cells. IDO is an immune checkpoint protein, thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation and antioxidant activity. Cancer cells are able to evade the immune system is by hijacking the checkpoint proteins. Increased IDO protein levels drive growth arrest and apoptosis of the effector T cells, a group of immune cells that mediate the immune system’s
ABI
lity to destroy pathogens. By reducing the number of effector T cells, IDO overexpression prevents the immune system from effectively destroying cancer cells. IDO overexpression has been observed in a wide range of human cancers such as prostatic, colorectal, pancreatic, cervical, gastric, ovarian, head or lung cancer. Physiological IDO activity has been implicated in T cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
BIOLOG
ical active IDO enzyme can be used for screening of IDO inhibitors, which have shown promising antitumor activity and appear to be synergistic in combination with chemotherapy and other forms of immunotherapy.