商品编号


YIF-LF-P0331



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

0.2 mg

商品信息


More Information



Product Details



Synonyms

H13; DFFA; DFF1; ICAD; DFF-45; Inhibitor of CAD; DNA Fragmentation Factor Subunit α; DNA Fragmentation Factor 45 kDa Subunit



Product Type

Protein



Properties



Source/Host

E. coli



Crossreactivity

Human



Purity

≥95% (SDS-PAGE)



Formulation

Lyophilized from 50mM NaH2PO4, pH8.0, 300mM NaCl, 130mM imidazole.



Other Product Data

Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by AbFrontier



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

After reconstitution, store at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





The inhibitor of caspase-3-activated DNase (ICAD) is a caspase 3 substrate that controls nuclear apoptosis. ICAD has two isoforms: a functional isoform of 45kDa, ICAD-L/DNA fragmentation factor (DFF) 45; and a 35kDa isoform, ICAD-S/DFF35. Although both ICAD-L and ICAD-S can bind and inhibit CAD, only ICAD-L was reported to be functional. ICAD is cleaved to be inactivated and allow caspase-activated DNase (CAD) to execute nuclear internucleosomal apoptotic DNA fragmentation. In non-apoptotic cells, CAD is complexed with its inhibitor, ICAD. The activation of the CAD/ICAD complex occurs through the caspase 3-mediated cleavage of ICAD at residues 117 and 224, which results in three ICAD fragments that are then released from CAD. In addition to its DNase inhibitory activity, ICAD acts as a CAD specific folding chaperone. There are recent reports that ICAD is a potential target for restoring a normal apoptotic signal transduction pathway in colon and brain cancer cells.


Product References
1) Enari M et al., (1998) Nature 391(6662):43-50. (General)
2) Sakahira H et al., (1999) J Biol Chem 274(22):15740-4. (General)
3) Charrier L et al., (2002) Cancer Res 62(7):2169-74. (General)
4) Fukushima K et al., (2002) J Mol Biol 321(2):317-27. (General)