商品编号


AG-40T-0269-C100



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 A; RAD6A; Ubiquitin-protein Ligase A; UBE2A



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human HR6A (Accession Nr. NP_003327) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Homolog of Rad6 A (HR6A), also known as ubiquitin-conjugating Enzyme E2A (UBE2A), is a ubiquitously expressed member of the ubiquitin-conjugating (E2) enzyme family that is highly expressed in the brain and heart. HR6A/UBE2A has a predicted molecular weight of 17 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. Like other E2 family members, HR6A/UBE2A has an E2 catalytic core domain that contains an active site cysteine residue required for the formation of a thioester bond with ubiquitin. HR6A/UBE2A is involved in many cellular processes including protein degradation, transcriptional regulation and the DNA damage response. HR6A/UBE2A functions with the UBR2 ubiquitin ligase (E3) to mediate ubiquitination of target substrates degraded through the N-end rule proteolytic pathway. HR6A/UBE2A also interacts with the human BRE1 E3 to ubiquitinate Histone H2B on Lys120, which promotes transcription via methylation of Histone H3 on Lys4. HR6A/UBE2A is activated via phosphorylation on Ser120 by CDK9 following exposure to UVC irr
ADI
ation and HR6A/UBE2A-RAD18-dependent ubiquitination of PCNA is required for DNA damage tolerance. HR6A/UBE2A also regulates the mRNA and protein levels of p53.