商品编号


AG-40T-0268-C250



品牌


Adipogen



公司


Adipogen



公司分类


Proteins



Size

250 ?g

商品信息


More Information



Product Details



Synonyms

UV Excision Repair Protein RAD23 Homolog A; Rad23A



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human HR23A Tandem UBA [TUBE1] (Accession Nr. NP_005044.1 ) fused to a His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES, 200mM NaCl, 1mM DTT pH 8.0.



Other Product Data


Use:
Allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His-specific antibodies. Use 50-100?g of protein to detect 2-25?g of purified K48-linked ubiquitin chains. The amount necessary for use in crude lysates needs to be determined empirically.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Human HR23A/Rad23A has two ubiquitin-associated (UBA) motifs that can each bind ubiquitin via a hydrophobic surface formed by residues located within the α1 and α3 helices of each UBA domain. Tandem-repeated ubiquitin binding entities (TUBEs), which consist of multiple tandem ubiquitin-binding UBA motifs have been developed for the isolation and identification of ubiquitinated proteins. TUBEs show increased affinity for poly-ubiquitin moieties compared to single UBA motifs. Additionally, TUBEs protect polyubiquitinated proteins from deubiquitylating enzymes, allowing for the detection of polyubiquitinated proteins at relatively low levels of abundance.