商品编号


P1020-25



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


Protein Expression / Purification Reagents



Size

25 g

商品信息

TCEP (tris(2-carboxyethyl)phosphine) is a strong reducing agent. Compared to dithiothreitol (DTT) and β-mercaptoethanol, TCEP has the advantages of being odorless, a more powerful reducing agent, an irrevers
IBL
e reducing agent, more hydrophilic, and more resistant to oxidation in air. It is soluble in water and stable at neutral pH. It does not reduce metals used in immobilized metal affinity chromatography such as Ni-NTA resin. TCEP is particularly useful when labeling cysteine residues with maleimides.

Note: Make 40 mL 1 M TCEP stock solution
1. Weigh 11.47 g TCEP powder. Dissolve in 25 ml molecular
BIOLOG
y grade water (stirring or shaking may be necessary). The resulting solution is at ~ pH 2.5.
2. Bring the solution to pH 7.0 with 10 N NaOH (prepared in molecular
BIOLOG
y grade water).
3. Bring to a final volume of 40 ml with molecular
BIOLOG
y grade water.
4. Aliquot and store at -20°C.

Additional Information






Product Name:

TCEP



Also Known As:

Tris (2-Carboxyethyl) phosphine Hydrochloride



Catalog No.:

P1020-25



Size:

25 g



CAS:

51805-45-9



Formula:

C
9
H
15
O
6
P ? HCl



Molecular Weight:

286.65 Da



Form:

Lyophilized powder



Quality Assurance:

> 95% by HPLC



Source:

Synthetic



Storage:

Elig
IBL
e for room temperature shipping. Store at 2-8°C upon receiving



PDF Data Sheet:

Download PDF datasheet



Image(s):

N/A



Shipping Method:

Room temperature shipping



References:

N/A




Details

TCEP (tris(2-carboxyethyl)phosphine) is a strong reducing agent. Compared to dithiothreitol (DTT) and β-mercaptoethanol, TCEP has the advantages of being odorless, a more powerful reducing agent, an irrevers
IBL
e reducing agent, more hydrophilic, and more resistant to oxidation in air. It is soluble in water and stable at neutral pH. It does not reduce metals used in immobilized metal affinity chromatography such as Ni-NTA resin. TCEP is particularly useful when labeling cysteine residues with maleimides.
Note: Make 40 mL 1 M TCEP stock solution 1. Weigh 11.47 g TCEP powder. Dissolve in 25 ml molecular
BIOLOG
y grade water (stirring or shaking may be necessary). The resulting solution is at ~ pH 2.5. 2. Bring the solution to pH 7.0 with 10 N NaOH (prepared in molecular
BIOLOG
y grade water). 3. Bring to a final volume of 40 ml with molecular
BIOLOG
y grade water. 4. Aliquot and store at -20°C.