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Innovative Research/Human PAI-1 N-terminal cysteine, active fraction/INTCYSPAI-A/1.0mg

天然蛋白
Innovative Research/Human PAI-1 N-terminal cysteine, active fraction/INTCYSPAI-A/1.0mg


商品编号


INTCYSPAI-A



品牌


Innovative Research



公司


Innovative Research



公司分类


Proteins



Size

1.0mg

商品信息

Product Description
Human PAI-1 N-terminal cysteine, active fraction
Wild type PAI-1 cloned with a cysteine residue at the N-terminus (patent pending). The active fraction is >95 percent pure and >95 percent active as determined by titration with HWM tc-urokinase and SDS PAGE. The protein is supplied in buffer with 1 mM DTT to prevent dimerization. To label with thiol reactive dye, buffer exchange into degassed 0.05M Sodium Phosphate; 0.1M NaCl; 1mM EDTA; pH 6.6 by desalting column. Collect into 10 fold molar excess of maleimide or iodoacetamide dye and incubate for 1 hour at room temperature. To preserve PAI-1 activity minimize the percentage of DMSO/DMF in the reaction. Remove free dye by desalting, ammonium sulfate precipitation, or dialysis.
References:

上一篇 Innovative Research/Human PAI-1 NBD labeled at the reactive center loop/IHPAI-P9NBD/0.5mg  下一篇 Innovative Research/Human PAI-1 N-terminal cysteine, active fraction/INTCYSPAI-A/0.5mg

产品货号:16179.2

16179.2 ¥
11至15个工作日送达