商品编号


AG-44T-0118-KI01



品牌


Adipogen



公司


Adipogen



公司分类


Assays



Size

1 Kit


商品信息


More Information



Product Details



Synonyms

MDM2 [HDM2] Ubiquitin Ligase Kit - p53 Substrate



Product Type

Kit



Properties



Application Set

Compound Screening



Crossreactivity

Human



Kit Contains

30μl of 10X E1 Enzyme
30μl of 10X E2 Enzyme (UBE2D3)
30μl of 10X GST-MDM2
30μl of 10X His-p53 Control Substrate
30μl of 10X Ubiquitin Solution
50μl of 10X Reaction Buffer
30μl of 10X Mg-ATP Solution
100μl of α-p53 Primary antibody
1ml of 5X Lo
ADI
ng Buffer
NOTE: Kit contains reagents sufficient for 10 x 30μl reactions and 5 Western Blots (mini-gel format).



Other Product Data


Use:
This kit is designed for
in vitro
ubiquitination of p53 by Mdm2. The ubiquitinated protein can be detected by Western blot using the supplied anti-p53 monoclonal antibody. This kit can be used as a positive control in studies that monitor the activities of other known or putative E3 ligase enzymes that ubiquitinate p53. Ubiquitinated p53 substrate can also be used to study E4 ligase activities of enzymes such as UBE4B, or the activity of deubiquitinating enzymes such as
USP
7.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -80°C.



Documents



Manual


Download PDF



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





MDM2 is a key regulator of p53 tumor suppressor protein activity and st
ABI
lity. MDM2 binds to and inhibits the transactivation domain of p53. In addition, MDM2 controls p53 st
ABI
lity by functioning as its E3 ligase in ubiquitination and by shuttling p53 from the nucleus to the cytoplasm for subsequent degradation. The importance of the p53/MDM2 relationship is underscored by the existence of an autoregulatory feedback loop whereby activated p53 transcriptionally upregulates the expression of its own inhibitor, MDM2.