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vectorlabs/VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 594 Anti-Rabbit (red)/DyLight 488 Anti-Mouse (gree

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vectorlabs/VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 594 Anti-Rabbit (red)/DyLight 488 Anti-Mouse (gree


商品编号


DK-8828



品牌


vectorlabs



公司


Vector Laboratories, Inc



公司分类





Size

1 kit

商品信息

Overview



Features:


Ready-to-use format?offers maximum convenience for fluorescence staining of cells and tissues

Robust cocktail formulation of DyLight
?
anti-mouse IgG/anti-rabbit IgG secondary antibodies

Will detect both mouse and rabbit primary antibodies with green and red DyLight fluorescent dyes in a single step

Affinity-purified, ultrapure, high affinity antibodies

Secondary detection reagent optimally labeled with DyLight
?
dyes to provide the brightest label for fluorescence microscopy?


Kit Contents:


15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking?

15 ml VectaFluor? Duet Reagent [DyLight
?
594 Anti-Rabbit IgG and DyLight
?
488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse, ready-to-use in 5% NHS.?


DyLight
?
488:


Excitation maximum: ? 493 nm

Emission maximum: ?518 nm

Color: ?Green


DyLight
?
594:


Excitation maximum: ? 593 nm

Emission maximum: ?618 nm

Color: ?Red


Product Citations:

View product citations for antibody DK-8828 on CiteAb

Additional Information






Catalog Number

DK-8828



Unit Size

1 kit



Country of Manufacture

United States



Applications

Immunofluorescence



Target Species

Universal (Mouse/Rabbit)



Conjugate

DyLight 488/DyLight 594



Color of Fluorescence

Kit (Green, Red)



Host Species

Horse



Format

Ready-to-Use







Additional Info


Details


VectaFluor? Duet Kit offers maximum convenience to achieve immunofluorescence double labeling in one step. This kit includes a ready-to-use detection reagent that will detect mouse and rabbit primary antibodies with green and red DyLight
?
fluorescent dyes in a single step. ?Our affinity purified, extensively cross-adsorbed secondary antibodies are conjugated to DyLight
??
dyes in a manner that ensures maximum degree of labeling without compromising antibody affinity or specificity. VectaFluor? Duet is compat
IBL
e with fluorescence staining of cells and tissues.

Kit Contents:


15 ml 2.5% Normal Horse Serum (ready-to-use) for blocking?

15 ml VectaFluor? Duet Reagent [DyLight
?
?594 Anti-Rabbit IgG and DyLight
?
488 Anti-Mouse IgG cocktail (rabbit-red, mouse-green)] made in horse, ready-to-use in 5% NHS.?


DyLight
?
488:


Excitation maximum: ? 493 nm

Emission maximum: ?518 nm

Color: ?Green


DyLight
?
594:


Excitation maximum: ? 593 nm

Emission maximum: ?618 nm

Color: ?Red


DyLight
?
?fluorescent dyes are direct alternatives to tr
ADI
tional fluorophores such as fluorescein (FITC) and rhodamine. The excitation and emission spectra parallel that of other commercially available fluorescent reagents allowing for easy substitution into an existing protocol without requiring any further instrumentation or filter sets. ?

DyLight
?
?dyes offer a number of potential advantages including greater photost
ABI
lity and brighter fluorescence. ?DyLight
?
?dyes are completely stable over a pH range of 4-9 making them compat
IBL
e with many aqueous-based buffers and diluents. The DyLight
?
?dyes can be applied as single labels or in combination with other DyLight
?
?dyes and fluorophores as part of a multiple immunofluorescent antigen staining methodology. ?The Dylight
?
?dyes currently offered are DyLight
?
?488 (green), DyLight
?
?549 (orange), DyLight
?
?594 (red), and DyLight
?
?649 (far red).

?



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