商品编号


PLK-1203



品牌


vectorlabs



公司


Vector Laboratories, Inc



公司分类


Antibody Labeling and Protein Labeling



Size

1 kit

商品信息

Overview



Features:


The ProtOn? HRP?Protein Labeling Kit is designed for simple and fast labeling of proteins with Horser
ADI
sh Peroxidase (HRP).

The kit is ideally suited to label antibodies or other proteins.

HRP is activated under precise conditions to generate?an optimal number of reactive aldehyde groups for subsequent conjugation with amine-containing molecules via reductive amination.

Conjugation of the HRP is accomplished through reaction with primary amine?on lysine residues or terminal amino groups on target proteins to form a stable secondary amine linkage.?


Kit Contents:

Each kit contains all the components needed for at least five labeling reactions.


Activated HRP Lyophilized Powder

Reaction Buffer (150 mM NaHCO3, pH 9.5 with preservative)

Reduction Buffer (5M Sodium Cyano- borohydride solution)

Quenching Buffer (1M Glycine with preservative)?


Each ProtOn? Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with HRP.

?

?Prostate (FFPE): ?Rabbit Anti-PSA primary antibody directly labeled with HRP using Vector ProtOn HRP Protein Labeling Kit (left) or using competitor TF HRP protein labeling kit (right). ?Note the more intense and crisp stain achieved when using the primary antibody labeled with Vector ProtOn HRP Protein Labeling Kit (left).

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Additional Information






Catalog Number

PLK-1203



Unit Size

1 kit



Country of Manufacture

United States



Target for Labeling

Protein



Required Reactive Group

Primary Amino Group



Tag/Group Incorporated

HRP (Peroxidase)







Additional Info


Details


Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn? Protein Labeling Kits simplify the attachment of biotin, fluorescein, or peroxidase (HRP) to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

The ProtOn
?
HRP Protein Labeling Kit is designed for simple and fast labeling of proteins with Horser
ADI
sh Peroxidase (HRP). The kit is ideally suited to label antibodies or other proteins. ?
HRP is activated under precise conditions to generate?an optimal number of reactive aldehyde groups for subsequent conjugation with amine-containing molecules via reductive amination. Conjugation of the HRP is accomplished through reaction with primary amine?on lysine residues or terminal amino groups on target proteins to form a stable secondary amine linkage.

Kit Contents:

Each kit contains all the components needed for at least five labeling reactions.


Activated HRP Lyophilized Powder

Reaction Buffer (150 mM NaHCO3, pH 9.5 with preservative)

Reduction Buffer (5M Sodium Cyano- borohydride solution)

Quenching Buffer (1M Glycine with preservative)?


Each ProtOn? Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with HRP.