商品编号


60621



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

2 vials

商品信息





Species: Human









Background:
The nuclear factor of activator T cells (NFAT) family of transcription factors plays an important role in immune response. T cell activation through the T cell synapse results in calcium influx. Increased intracellular calcium levels activate the calcium-sensitive phosphatase, calcineurin, which rapidly dephosphorylates the serine-rich region (SRR) and SP-repeats in the amino termini of NFAT proteins. This results in a conformational change that exposes a nuclear localization signal promoting NFAT nuclear import. In the nucleus, NFAT proteins cooperate with other proteins to bind to DNA.




Description:
The
NFAT Reporter
– Jurkat Cell Line contains a firefly luciferase gene under the control of the NFAT response element stably integrated into Jurkat cells. The NFAT Reporter cell line has been validated for response to thapsigargin, ionomycin, and phorbol 12-myristate 13-acetate (PMA). It is useful as a control cell line for other NFAT reporter cell lines expressing various immune checkpoint receptors.




Materials Required But Not Provided:
PMA (
LC Laboratories
#P1680): Prepare stock solution in DMSO.
Ionomycin (Sigma #I3909): Prepare stock solution in DMSO.
Thapsigargin (Sigma #T9033): Prepare stock solution in DMSO.
Assay medium: Thaw Medium 2 (BPS Cat. #60184)
96-well tissue culture plate or 96-well tissue culture-treated white clear-bottom assay plate
One-Step? luciferase assay system (BPS Cat. #60690) for measuring firefly luciferase activity
Luminometer




Synonym(s):
Nuclear factor of activated T cells; T cell




Supplied As:
Each vial contains ~2x10^6 cells in 1 ml of 10% DMSO.




Mycoplasma Testing:
The cell line has been screened using the PCR-based VenorGeM? Mycoplasma Detection kit (
Sigma-Aldrich
) to confirm the absence of Mycoplasma species.




Instructions for use:
See data sheet for detailed culturing and assay protocol




Storage / St
ABI
lity:
Immediately upon receipt, store vials in liquid nitrogen.




Application(s):
? Monitor intracellular calcium levels. ? Screen for activators or inhibitors of PKC/ Ca2+ pathway. ? Screen for ago
NIST
s or antago
NIST
s of T cell receptors. ? Control for immune checkpoint NFAT reporter cell lines.




Reference(s):

1. Clipstone NA, Crabtree GR.
Nature.
1992 Jun 25;
357(6380)
:695-7.
2. Lyakh, L.,
et al. Mol Cell Biol.
1997 May;
17(5)
:2475-84.

Application Reference(s):
1.?
Interleukin 2 Secretion by T Cells for Detection of
BIOLOG
ically Active Staphylococcal Enterotoxin Type E?
(2017)




Notes:

Contact us for pricing.

License Disclosure
:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.