商品编号


60481



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

2 vials

商品信息













Background:
Interleukin-2 (IL-2) is a key cytokine important for proliferation and differentiation of T cells into effector T cells through interaction with the IL-2 receptor.




Description:
Human IL-2 reporter construct is stably integrated into the genome of Jurkat T-cells. The firefly luciferase gene is controlled by a human IL-2 promoter.

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Materials Required But Not Provided:
Thaw Medium 2 (BPS Cat. #60184)
ONE-Step Luciferase Detection Reagent (BPS Cat. #60690-1)
PMA (Phorbol 12-Myristate 13-Acetate, Fisher, # BP685-1), 1 mg/ml stock in DMSO
Ionomycin (Fisher, #BP25271, 1 mg/ml stock in DMSO
Anti-human CD3 antibody (BPS Cat. #71274)
Anti-CD28 antibody (Clone 28.2,
BD
Biosciences, #555725)
TCR Activator/Raji Recombinant Cells (BPS Cat. #60556)




Host Cell line:
Jurkat




Mycoplasma Testing:
This cell line has been screened using the MycoAlert? Mycoplasma Detection Kit (Cat. No. LT07-118) to confirm the absence of Mycoplasma contamination. MycoAlert Assay Control Set (Cat. No. LT07-518) was used as a positive control.




Format:
Each vial contains ~2 x 10^6 cells in 1 ml of 10% DMSO in FBS.




Storage / St
ABI
lity:
Store in liquid nitrogen immediately upon receipt.




Application(s):
The IL-2-luciferase Jurkat reporter cell line is suitable for monitoring the transcription activity of IL-2 in response to stimulants, and establishing cell-based screens for inhibitors that target specific IL-2 stimulating molecules.




Reference(s):
1. Weaver JR et.al. (2007) Characterization of the sequence and architectural constraints of the regulatory and core regions of the human interleukin-2 promoter. Mol. Immunol. 44: 2813-2819.
2. Hughes CCW and Pober JS (1996) Transcriptional Regulation of the Interleukin-2 Gene in Normal Human Peripheral Blood T Cells. J. Biol. Chem. 271: 5369-5377.




Notes:
License Disclosure:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Scientific Category:
Immunotherapy




Product Type:
Cell Line




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Contact us
for specific information on other lots.