商品编号


MB-9002



品牌


vectorlabs



公司


Vector Laboratories, Inc



公司分类


Nucleic Acid Labeling



Size

1 kit

商品信息

Overview



Features:


Enables simple and uniform labeling of blunt, overhanging or recessed 3' ends of the DNA.?

Choice of diverse labels - fluorophores, biotin, haptens, affinity ligands (not included)


?

Kit components:

The?3' EndTag? Labeling Kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:


Terminal Transferase (TdT)

SH-GTP

10x TdT buffer

Precipitant


?

Note:
?Requires thiol-reactive labeling reagent:


Biotin (Long Arm) Maleimide?

DNP Maleimide

Fluorescein (Long Arm) Maleimide

Fucose Maleimide

Texas Red
?
Maleimide


Note on Shipping
: This item is shipped on ice requiring additional shipping charges.

Additional Information






Catalog Number

MB-9002



Unit Size

1 kit



Country of Manufacture

United States



Target for Labeling

DNA



Required Reactive Group

3' -OH group of DNA



Tag/Group Incorporated

Thiol







Additional Info


Details


End labeling is a favored method for applications where an internal label might interfere with hybridization or sequence specific protein binding.
5' or 3' EndTag? labeled nucleic acids can be used for applications such as DNA hybridization, PCR,
in situ
hybridization, the binding of capture probes to affinity matrices, or electrophoretic mobility shift assays (EMSA). Short oligonucleotides are labeled more efficiently with these systems than with other methods. In addition, end labeling of oligonucleotides is an economical alternative to having labels inserted during synthesis.
Both the 5' EndTag? and the 3' EndTag? Nucleic Acid Labeling Systems enable the covalent attachment of a variety of fluorescent dyes, haptens, or affinity tags to the respective ends of the nucleic acids using thiol-specific chemistry. Labels containing thiol-reactive groups (maleimides, iodoacetamides, etc.) can easily be incorporated.
Labeling time is about 1 hour with very little hands-on time.

3' EndTag? Labeling Kit

The 3' EndTag? System enables simple and uniform labeling of blunt, overhanging, or recessed 3' ends of DNA. 3' end labeling is the preferred method if the terminal phosphate at the 5' end must be preserved.

Labeling using the?3' EndTag? Labeling Kit is achieved in two steps:

1. Terminal deoxynucleotidyl transferase (TdT) incorporates the thiol-containing nucleotide (SH-GTP) onto the 3' end.
2. Thiolated DNA is coupled with a thiol-reactive label.

Thiol-reactive label is not included in the kit and should be selected separately.


Kit Contents:

The kit is designed to perform 20 labeling reactions of up to 0.5 nmols of 3' ends (e.g. about 4.2 μg of a 25 base oligonucleotide) per reaction and includes:


terminal transferase (TdT)

SH-GTP

10x TdT buffer

precipitant


Note on Shipping:
This item is shipped on ice requiring additional shipping charges.