商品编号


90236-B



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cytokine(s)



Size

10 ?g

商品信息





Species: Mouse

Host Species:
E. coli


MW: 23 kDa





Genbank #: P20826

a.a: 26–189





Background:
SCF is a stromal cell-derived cytokine synthesized by fibroblasts and other cell types. SCF promotes proliferation and early differentiation of cells at the level of multipotential stem cells. It has been suggested that SCF is essential for optimal production of various hematopoietic lineages, mainly because of its
ABI
lity to prevent apoptosis when it co-stimulates with other cytokines. The receptor for SCF, designated SCFR(CD117), is the oncogene designated as KIT. The
BIOLOG
ical activities of SCF are synergised considerably by colony stimulating factors GM-CSF and G-CSF, and also by IL-7, Epo and some other growth and differentiation factors. In combination with IL-7, SCF stimulates the proliferation of pre-B-cells. SCF is also a potent chemoattractant for cells, for example, mast cells, expressing the kit receptor. One response to SCF in these cells is a characteristic rearrangement of the actin filaments of the
Cytoskeleton
.




Description:
Recombinant SCF (Kit Ligand) is a disulfide-linked monomer protein consisting of 165 amino acid residues, and due to glycosylation migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding murine SCF mature chain was expressed in
E. coli
.




UniProt
P20826




Synonym(s):
Kit Ligand, SCF, Stem Cell Factor, Steel Factor, MGF, and Mast Cell Growth Factor, KITL, CD117L




Purity:
≥95% by SDS-PAGE and HPLC




Endotoxin Level:
<0.1 ng/?g (1 EU/?g), using the LAL gel clot method.




BIOLOG
ical Activity:
The ED
50
was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is <2.0 ng/ml, corresponding to a specific activity of > 5 x 10
5
units/mg.




Formulation:
Lyophilized from a 0.2 ?m filtered solution in 20 mM Tris, 5% trehalose, pH 7.5.




Format:
lyophilized protein




Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.




Storage / St
ABI
lity:

The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.




Reference(s):
1. Bedell MA, et al. J Androl. 2004 Mar-Apr,25(2):188-99.
2. Heissig B, et al. Thromb Haemost. 2003 Oct,90(4):570-6.
3. Wehrle-Haller B. Pigment Cell Res. 2003 Jun,16(3):287-96.




Warning(s):
Avoid freeze/thaw cycles




Amino Acid Sequence:
KEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA




Scientific Category:
Cytokine/Growth Factors




Product Type:
Recombinant Protein




Data shown is lot-specific.
Contact us
for specific information on other lots.