商品编号


TG4040



品牌


TopoGEN



公司


TopoGEN



公司分类


Kit replenishments



Size

1 ml (A), 1 ml (B)

商品信息


Topo II Buffer is supplied as a 10x Stock solution in two parts that must be mixed prior to use:


10X Incomplete Topo II Assay Buffer A contains the following: 0.5M Tris-HCl (pH8.0), 1.5M NaCl, 0.1M MgCl2, 5 mM dithiothreitol.

10X ATP Buffer B contains 20 mM ATP in water.


These two buffers must be mixed to prepare a final 5X working stock of assay buffer.? Fur
Thermo
re, the final 5X Complete Buffer contains both A and B and should be made fresh for each experiment.? Do not freeze the 5X Complete Buffer; it is stable for 24 hours on ice.? To Prepare the 5X Complete Buffer, mix equal volumes of A and B (0.1ml of A and 0.1ml of B) and place at 4° C or on ice.


Quality Control Analysis:
The 5X Complete Topo II Assay Buffer (containing equal parts A and B above) is optimized and tested for decatenation and relaxation activity of eukaryotic topo II.? The Complete Buffer should be made fresh for each
experiment.