商品编号


TG1015-1



品牌


TopoGEN



公司


TopoGEN



公司分类


Topoisomerase assay kits



Size

100 Assays

商品信息




Topoisomerase I Assay Kit Product Description
This kit contains reagents for routine detection of type I

topoisomerase activity. DNA
Marker
s (included) allow unequivocal detection of the enzyme. The assay is specific for topoisomerase I; (eukaryotic type II topoisomerases require MgCl2 and ATP). The standard topo I assay kit is supplied with EDTA containing reaction buffer (minus Mg++) in order to prevent nucleolytic nicking of the supercoiled substrate (although we note that some eukaryotic type I activities are stimulated by Mg++). This assay will work even with crude extracts. A detailed manual of instruction (see link above) is included that describes how to run the gels to maximally resolve topoisomers for analysis of DNA unwinding.



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Topoisomerase I Assay Kit Contents (representative for 100 assay kit size):

-Supercoiled pHOT-1 DNA (25 ?g in 100 ?l TE buffer, or 0.25 ?g/ul)
-Relaxed pHOT-1 DNA
Marker
, relaxed by topo I (50 ul at 0.05 ?g/ml in gel lo
ADI
ng buffer)
-10X Topo I assay buffer (300 ?l)
-5X Stop buffer/gel lo
ADI
ng dye (600 ?l)
-Detailed instruction manual
-Sample data and documentation of all controls


Reviews and Citations:


Dexheimer TS, Pommier Y:
DNA cleavage assay for the identification of topoisomerase I inhibitors.
?
Nature
2008, 3(11): 1736-1750. doi:10.1038/nprot.2008.174

Zhang L, Sullivan P, Suyama J, et. al:
Epidermal growth factor-induced heparanase nucleolar localization augments DNA topoisomerase I activity in brain metastatic breast cancer.
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Molecular Cancer Research
2010, 8: 278-290. doi: 10.1158/1541-7786.MCR-09-0375