商品编号


71249



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Immunotherapy



Size

2 vials

商品信息













Background:
CD47 (also known as Rh-associated protein, GP42, integrin-associated protein (IAP), and neurophilin,) is an immunoglobulin-like protein that interacts with its receptor, Signal-regulatory protein alpha (SIRPα), on macrophages. This binding interaction regulates transmigration, oxidative burst cytokine production, and phagocytosis, creating a “don’t eat me” signal. CD47 is ubiquitously expressed on the surface of normal cells, but is overexpressed in numerous cancer cells, where it is thought to contribute to the resistance of tumors to phagocyte-dependent clearance.




Description:
Recombinant HEK293 cell line over-expressing full length human CD47 (NM_198793.2). Surface expression is confirmed by flow cytometry.




Synonym(s):
Rh-associated protein, GP42, integrin-associated protein (IAP), neurophilin




Host Cell line:
Human Embryonic Kidney cell line (HEK293). Adherent epithelial cells.




Growth Media:

Thaw Medium 1 (BPS CAT. # 60187):
?MEM medium (Hyclone #SH30024.01) supplemented with 10% FBS (
Invitrogen
#26140-079), 1% non-essential amino acids (Hyclone #SH30238.01), 1 mM Na pyruvate (Hyclone #SH30239.01), 1% Penicillin/Streptomycin (Hyclone SV30010.01

Complete Growth Medium:
Thaw Medium 1 (BPS Cat. #60187) plus 100 ?g/ml Hygromycin B (
Thermo
Fisher, Cat. #10687010).




Format:
Each vial contains ~3 x 10^6 cells in 1 ml of 10% DMSO in FBS.




Storage / St
ABI
lity:
Store in liquid nitrogen immediately upon receipt.




Application(s):
Ideal for protein binding analyses and studying SIRPα receptor activity.




Data shown is lot-specific.
Contact us
for specific information on other lots.