商品编号


79377



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


New Products



Size

96 reactions


商品信息













Background:
Programmed Cell Death Protein 1 (PD-1) is a receptor which is expressed on activated T-cells. Binding of PD-1 to its ligands, PD-L1 and PD-L2, negatively regulates immune responses. The PD-1 ligands are found on most cancers, and PD-1: PD-L1/2 interaction inhibits T cell activity and allows cancer cells to escape immune surveillance.




Description:
The PD-1/PD-L1 Inhibitor Screening Cell-Based Assay is a bioluminescent cell-based assay that can be used to screen and profile inhibitors of the PD-1:PD-L1 interaction. The assay consists of two main components:

? Growth-Arrested PD-1 Effector cells (PD-1/NFAT reporter-Jurkat cells): Reporter Jurkat T cells expressing firefly luciferase gene under the control of NFAT response elements and also constitutively expressing human PD-1. These cryopreserved cells are provided in a thaw-anduse format that does not require cell propagation. These cells are modified not to be expanded and are intended to be used in a single experiment.

? Expression vectors for TCR activator, and Human PD-L1: Transfection-ready vectors are used to transfect cells to create the target cells that overexpress PD-L1 and an engineered cell surface T cell receptor (TCR) activator.




Materials Required But Not Provided:
? HEK293 cell and its growth medium (Thaw medium 1, #60187) or other cell lines

? Transfection reagent for mammalian cell line [We use Lipofectamine? 2000 (Life technologies #11668027). However, other transfection reagents work equally well.]

? Opti-MEM I Reduced Serum Medium (Life technologies #31985-062)

? 96-well tissue culture-treated white clear-bottom assay plate

? Luminometer





Synonym(s):
Programmed Cell Death 1 Ligand 1, PDL1, PD-L1, B7 homolog 1, B7H1, B7-H1, CD274, PDCD1L1




Supplied As:
Each kit contains sufficient reagents for 100 assays using a 96-well plate.




Format:

COMPONENTS:






Instructions for use:
See assay kit data sheet for detailed protocol.




Storage / St
ABI
lity:
Store cell line in liquid nitrogen immediately upon receipt. Store other components as described below. Stable for at least 6 months from date of receipt when stored as directed.




Application(s):

? Screen for activators or inhibitors of PD-1 signaling in a cellular context

? Characterize the
BIOLOG
ical activity of PD-1 and its interactions with ligands




Warning(s):
Avoid freeze/thaw cycles.




Scientific Category:
Immunotherapy




Product Type:
Assay Kit




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Contact us
for specific information on other lots.