商品编号


20060



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Molecular Biology Tools



Size

100 reactions

商品信息













Background:
The Quick PCR? Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without time-consuming ligation steps. Multiple PCR inserts can be added in a single step. The procedure is easy: a primer set homologous to the gene of interest and the vector are used to amplify a PCR product. This purified PCR product is incubated with restriction enzyme-digested vector and Reagent A of the Quick PCR? Cloning Kit. Following a brief (5 minute) incubation with Reagent B, the annealed vector is ready for transformation.




Description:
The BPS Bioscience Quick PCR? cloning kit allows for the insertion of multiple DNA fragments into the desired vector in a single reaction. No ligation step is required, saving time and effort. The method is fast, simple, and efficient. Go from PCR product to transforming cells in under an hour! Simply mix the purified PCR product with the vector in a 1:1 molar ratio (Total DNA : Vector) and follow the protocol. Efficiencies of >90% are routinely obtained. Available with or without competent cells for even greater efficiency and flexibility.




Supplied As:

Components:
Reagent A
Reagent B
Buffer A
10X BSA (0.1% w/v)
Quickgrow?
E. coli
competent cells




Efficiency:
Efficiencies of >90% are typical.




Format:
assay kit




Instructions for use:
See data sheet for detailed protocol.




Storage / St
ABI
lity:

Store competent cells at -80°C upon receipt, all other components may be stored at -20°C or -80°C. Avoid freeze/thaw cycles. Upon first thaw, store all reagents in single use aliquots. Stable for at least 6 months from date of receipt, when stored as directed.




Application(s):
Kit is designed for cloning any gene or DNA fragment into any vector in a quick, single-step reaction. Can be used with multiple inserts in a single reaction. Also suitable for fixing or adding point mutations, insertions, or deletions.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Mol.Biol.Reag./Cloning




Product Type:
PCR Kit




Data shown is lot-specific.
Contact us
for specific information on other lots.