商品编号


20000



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Molecular Biology Tools



Size

20 x 50 ?l

商品信息













Description:
Quickgrow?
E. coli
is specifically selected for its fast growth properties. Unlike some other commercial
E. coli
strains, which take at least 14 hrs for the colonies to grow large enough for picking, Quickgrow?
E. coli
can grow to pickable colonies on LB plates within 9 hours. Quickgrow?
E. coli
provide alpha-complementation of the beta-galactosidase gene for color selection. They may be used for producing single-stranded DNA from M13 or phagemid and are also useful for induc
IBL
e protein expression of those genes under LacUV5 promoter control. The genotype is F' proA+B+ lacIq D(lacZ)M15/ D(lac-proAB) glnV thi-1 D(hsdS-mcrB)5.




Supplied As:

Components:
20 vials (50 ?l each) Quickgrow?
E. coli
cells, ~1 x10
9
cells/vial)
10 ?l pUC18 control DNA (0.1 ng/?l)
10 ml SOC medium




Efficiency:
1.0 x 10
9
CPU (colonies per ?g pUC18 DNA)




Assay Conditions:
Transformations are performed with 50 aliquots of cells and 2 ul of pUC18 control plasmid (10 pg/ul) following the provided protocol. Dilute the reaction 1:100 with Super Optimal Broth with 20 mM glucose (SOC) at room temperature. 100 ul of the dilution is plated in duplicate on LB agar plates with 50 mg/ml ampicillin. The plates are incubated at 37°C overnight and the efficiency is calculated based on the average number of colonies per plate.




Format:
Aqueous buffer solution




Instructions for use:
See data sheet for detailed protocol.




Storage / St
ABI
lity:

Store competent cells at -80°C upon receipt. Avoid freeze/thaw cycles. Upon first thaw, store in single use aliquots. Stable for at least 6 months from date of receipt, when stored as directed.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Mol. Biol. Competent Cells / Buffer




Product Type:
Cells




Data shown is lot-specific.
Contact us
for specific information on other lots.