商品编号


60506



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Cell Surface Receptor(s)



Size

2 vials

商品信息





Species: Human





Genbank #: P13569





Background:
Cystic fibrosis transmembrane conductance regulator (CFTR) is a protein that in humans is encoded by the CFTR gene. CFTR is an ABC transporter-class ion channel that transports chloride and thiocyanate ions across epithelial cell membranes. Mutations of the CFTR gene affect functioning of the chloride ion channels in these cell membranes, le
ADI
ng to cystic fibrosis. It is characterized by the triad of chronic bronchopulmonary disease (with recurrent respiratory infections), pancreatic insufficiency (which leads to malabsorption and growth retardation) and elevated sweat electrolytes.




Description:
The CFTR-HEK293 Cell Line stably expresses full length, human cystic fibrosis transmembrane conductance regulator (CFTR) protein (GenBank # P13569). A synthetic codon-optimized DNA sequence encoding human CFTR protein with C-terminal Streptavidin-Binding Peptide (SBP) tag is stably integrated in 293HEK-Trex cells.




Synonym(s):
CFTR, cystic fibrosis transmembrane conductance regulator




Supplied As:
Each vial contains ~2 X 10
6
cells in 1 ml of Sigma Freezing Medium (Cat. # C-6164).




BIOLOG
ical Activity:
The expression of CFTR is confirmed by Western blotting and flow cytometry (FACS).




Host Cell line:
HEK293 cells




Instructions for use:
See cell line data sheet for detailed culturing and assay protocol.




Storage / St
ABI
lity:

Store in liquid nitrogen immediately upon receipt. Cells are stable after a minimum of seven continuous passages. Upon first use, amplify the cells in culture and make several frozen aliquots for future use.




Application(s):
?Drug compound screening for activators or inhibitors of CFTR
?Functional assays for CFTR
?Efficient antigen for mouse immunization




Reference(s):
1. Gadsby, D.C., et al. Nature 440 (7083): 477–483 (2006).
2. Hillier, L.W., et al. Nature 424:157-164 (2003).
3. McCann, C. M., et al. BioTechniques 38 (6):945–952 (2005).




Notes:

Contact us for pricing.

License Disclosure:
Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
ion channel




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.