商品编号


27462



品牌


Bpsbioscience



公司


BPS Bioscience Inc.



公司分类


Biotinylation



Size

10 vials

商品信息





Species: E. coli









Background:
Biotin-labeling is commonly used for non-r
ADI
oactive labeling and purification of proteins and other target molecules. Biotin labeling takes advantage of the exceptionally strong interaction between biotin (vitamin H) and either avidin or streptavidin. The affinity of biotin to avidin or streptavidin is one of the strongest known non-covalent interactions of a protein and ligand, exhibiting a dissociation constant (Kd) around 10-15. BirA protein ligase covalently adds biotin to biotin-acceptor peptides containing an AviTagTM sequence in a highly efficient and specific ATP-dependent reaction.




Description:
BirA-transformed Competent E. coli cells are supplied as 10 x 50 μl vials. Strain BL21, a chemically competent E. coli B strain, contains an IPTG-induc
IBL
e BirA expression plasmid and constitutively-expressed streptomycin/spectinomycin resistance gene. These cells are compat
IBL
e with most cloning vectors and are suitable for expression and biotinylation of recombinant proteins using AviTagTM technology.




Supplied As:
10 x 50 μl vials




Host Cell line:
E. coli




Instructions for use:
For a detailed protocol, please see BPS Bioscience’s E. coli in vivo Biotinylation Kit, Cat. #27641.




Storage / St
ABI
lity:
One year from date of receipt when stored at -80°C.




Application(s):
Useful for specific labeling of proteins/peptides with biotin for downstream applications.




Notes:

Contact us for pricing.

License Disclosure
:Purchase of this cell line grants you with a 10-year license to use this cell line in your immediate laboratory, for research use only. This license does not permit you to share, distribute, sell, sublicense, or otherwise make the cell line available for use to other laboratories, departments, research institutions, hospitals, universities, or biotech companies. The license does not permit use of this cell line in humans or for therapeutic or drug use. The license does not permit modification of the cell line in any way. Inappropriate use or distribution of this cell line will result in revocation of the license and result in an immediate cease of sales and distribution of BPS products to your laboratory. BPS does not warrant the suit
ABI
lity of the cell line for any particular use, and does not accept any li
ABI
lity in connection with the handling or use of the cell line. Modifications of this cell line, transfer to another facility, or commercial use of the cells may require a separate license and additional fees; contact sales@bp
SBI
oscience.com for details. Publications using this cell line should reference BPS Bioscience, Inc., San Diego.




Warning(s):
Avoid freeze/thaw cycles




Scientific Category:
Molecular & Cell
BIOLOG
y




Product Type:
Cell Line




Data shown is lot-specific.
Contact us
for specific information on other lots.