商品编号


M114



品牌


Quidel



公司


Quidel,inc.



公司分类


Lyra



Size

96 tests

商品信息

Description:
Assay Kit
Features & Benefits:






The Lyra Parainfluenza Virus Assay is a multiplex real-time PCR assay for the qualitative detection and identification of viral nucleic acids extracted from nasal swabs and nasopharyngeal swabs from symptomatic patients. The assay has been shown to detect and differentiate parainfluenza virus types 1, 2 and 3.

During the test, a multiplex RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for each of the target viruses present in the sample. Identification of PIV-1, PIV-2, PIV-3 and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the genomes of PIV-1, PIV-2, PIV-3 and the PRC.

The Lyra Parainfluenza Virus Assay has a convenient, one-step reagent set up performed at room temperature on the bench top, without the need for ice or other cooling accessories.? Moreover, optimized cycling conditions make results available in less than 75 minutes following extraction to aid in timely diagnosis and treatment. Lyra Parainfluenza Virus RT-PCR is available for use on the
Applied Biosystems
?
7500 Fast Dx Real-Time PCR Instrument.

The Lyra Parainfluenza Virus Assay provides? the flexibility to ?customize respiratory panels with other Lyra Real-Time PCR respiratory assays (Lyra Influenza A+B, Lyra hMPV+RSV, Lyra Adenovirus) that are run under the same cycling conditions in order to adapt to the ?shifting demands caused by ?variable local or seasonal disease prevalence.










Feature
Benefit



One-step reagent set-up
Rehydration solution is simply added to the lyophilized Master Mix



Speed
Results in less than 75 minutes after extraction



Easy training
Simplified and uniform workflow with standard pipetting volumes



Refrigerated storage
No freezer needed. 2°C to 8°C storage



Long shelf life
2 years from date of manufacture



Room temperature set up
No ice or cooling block required



Flex
IBL
e 96-test format
Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls



Multi-well multiplex panels
Standardized
Thermo
cycling conditions for the respiratory assays allow for multiple analytes to be analyzed on the same plate from the same extracted specimen, effectively providing a customizable panel of results












Product Specifications:




Product Spec Name
Product Spec Data



Sample type
Nasal swab, Nasopharyngeal swab



Time to results
Less than 75 minutes after extraction



Reagent storage conditions
2°C to 8°C



Controls storage conditions
2°C to 8°C



Sensitivity*
PIV-1: 100%; PIV-2:100%; PIV-3:100%



Specificity*
PIV-1: 99.8%; PIV-2:100%; PIV-3:99.6%



Positive Percent Agreement*
PIV-1: 100%; PIV-2:100%; PIV-3:100%



Negative Percent Agreement*
PIV-1: 98.8%; PIV-2:94.0%; PIV-3:100%



LOD
PIV-1: 2.50 x 10
0
; PIV-2: 2.50 x 10
2
; PIV-3:8.00 x 10
1
(TCID
50
/mL)



Sample volume needed for extraction
180 ?L



Purified nucleic acid volume needed to testing
5 ?L



Shelf life
24 months








*Refer to Package Insert for additional performance claims.