商品编号


M103



品牌


Quidel



公司


Quidel,inc.



公司分类


RSV



Size

96 tests

商品信息

Description:
Assay Kit
Features & Benefits:






?

The Lyra RSV + hMPV Assay is a multiplex real-time PCR assay for the qualitative detection and identification of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV) viral RNA extracted from nasal swabs, nasopharyngeal swabs, and nasal aspirate/wash specimens. This real time RSV + hMPV test detects RSV and hMPV and is able to differentiate between them.

A multiplex real-time RT-PCR reaction is carried out under optimized conditions in a single tube generating amplicons for RSV, hMPV and the Process Control (PRC). Identification of RSV and hMPV and the PRC occurs by the use of target-specific primers and fluorescent-labeled probes that hybridize to conserved regions in the NS2 and polymerase genes of RSV and polymerase gene of hMPV and the PRC.

The Lyra RSV + hMPV Assay has a one-step reagent set up. Test results are available in less than 75 minutes after extraction. Lyra RSV + hMPV RT-PCR is available for use on the
Applied Biosystems
?
7500 Fast Dx, QuantStudio
TM
Dx Real-Time PCR Instrument and Cepheid
?
SmartCycler
?
II.

The Lyra RSV + hMPV Assay is one in a growing menu of tests and intended to aid in the differential diagnosis of RSV and hMPV infections in humans.










Feature
Benefit



One-step reagent set-up
Rehydration solution is simply added to the lyophilized Master Mix



Speed
Results in less than 70?minutes after extraction



Easy training
Simplified and uniform workflow with standard pipetting volumes



Refrigerated storage
No freezer needed. 2°C to 8°C storage



Long shelf life
2 years from date of manufacture



Room temperature set up
No ice or cooling block required



Flex
IBL
e 96-test format
Kit includes vials of 12x8 Master Mix, Rehydration Solution and liquid Process Controls












Product Specifications:




Product Spec Name
Product Spec Data



Sample type
Nasal swab, Nasopharyngeal swab



Time to results
Less than 75 minutes after extraction



Reagent storage conditions
2°C to 8°C



Controls storage conditions
2°C to 8°C



Sensitivity*
RSV: 98.6%



Specificity*
RSV: 96.8%



Positive Percent Agreement*
hMPV: 98.0%



Negative Percent Agreement*
hMPV: 98.9%



LOD
RSV A: 6.29E-01; RSV B: 7.5E-01; hMPV-A1: 1.7E+01; hMPV-A2: 2.91E+00; hMPV-B1: 1.05E+00; hMPV-B2: 2.25E+00



Sample volume needed for extraction
180 ?L



Purified nucleic acid volume needed to test
5 ?L



Shelf life
24 months








*Data for
Applied Biosystems
? 7500 Fast Dx.?Refer to Package Insert for additional performance claims.