商品编号


MC1061-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


Competent Cells



Description

10x100 μl (10 tubes), >1.0x108


Size

1 Ea

商品信息

Chemically competent
E. coli
cells with high st
ABI
lity suitable for high efficiency transformation.




Description:

Chemically competent
E. coli
cells with high st
ABI
lity suitable for high efficiency transformation.
For research use only.

Parent of DH10B/TOP10 and derived strains.


Application:

For cloning, sub-cloning and phage display, but cannot be used for reinfection.

Transform efficiency is >1.0x10
8
cfu/?g with pUC19 control DNA.


Genotype:
?
F-?Δ(
ara-leu
)
7697
[
araD139
]B/r?Δ(
codB
-
lacI
)3
galK16

galE15
λ-?e14-?
mcrA0

relA1

rpsL150
(StrR)
spoT1

mcrB1

hsdR2
(r-m+)


Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze



Competent Cell Type:
Chemically



Transformation Efficiency (cfu/μg):
>1.0x10
8
cfu/?g with pUC19 control DNA


Blue-White Screening:
Yes, IPTG induction required


Strain:
K12


Reduces Recombination:
Yes


Cloning Methylated DNA:
Yes


Improves Plasmid Quality:
Yes


Preparing Unmethylated DNA:
Not suitable


T1 Phage Resistant:
No