商品编号


JM-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


Competent Cells



Description

10x100μl (10 tubes), >1x10?


Size

1 Ea

商品信息

High yields, high efficiency, no heat shock necessary.




Description:
High st
ABI
lity, high efficiency, no heat shock necessary. For Research Use Only.
Application:

Ideal for sub-cloning, single strained DNA, high quality plasmid preparation, and library construction

JM109 is a K strain bacterium that provides minimized recombination and aids in plasmid st
ABI
lity which results in high quality plasmid DNA preparation. The strain carries the hsdR17 genotype, which prevents cleavage of heterologous DNA by an endogenous endonuclease. JM109 strain supports Alpha-complementation for blue/white screening for recombinant plasmids. The presence of the F' factor also allows growth of bacteriophage M13 vectors for rescue of single stranded DNA. Transform efficiency is around?>1x10
9
?cfu/?g with pUC18 control DNA.


Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Recommended reaction conditions:

Mix DNA with competent cells

Let stand on bench for 5 minutes

Direct plate


Genotype:
F' (traD36, proAB+, lacIq, lacZ-M15),endA1, recA1, hsdR17, (rk-, mk+), mcrA, supE44, e-gyrA96, relA1, -(lac-proAB)
Reference:

Yanisch-Perron, C.; Vieira, J.; and Messing, J. Gene, (1985) 33, 103-119


Competent Cell Type:
Chemically


Transformation Efficiency (cfu/μg):
>1x10
9


Blue-Whiet Screening:
Yes


Strain:
K12


Reduces Recombination:
Yes


Cloning Methylated DNA:
No


Improves Plasmid Quality:
Yes


Preparing Unmethylated DNA:
Yes


T1 Phage Resistant:
No


RecA Deficient:
Yes