商品编号


HB-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


Competent Cells



Description

10x100μl (10 tubes), >1x10?


Size

1 Ea

商品信息

High yields, high efficiency, no heat shock step.




Description:
High st
ABI
lity, high efficiency, no heat shock step. For Research Use Only.


Application:

Ideal for sub-cloning and scale-up applications

HB101 strain is a hybrid K12 x B bacterium, containing the recA13 mutation that minimizes recombination and aidsin insert st
ABI
lity. In addition, it carries the hsdS20(rB-mB-) restriction minus genotype which prevents cleavage of cloned DNA by endogenous restriction enzymes. HB101 strain does not support Alpha-complementation for blue/white screening. Transform efficiency is around 1.0x10
7
~ 1.0x10
9
cfu/?g with pUC18 control DNA.


Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.


Recommended reaction conditions:

Mix DNA with competent cells

Let stand on bench for 5 minutes

Direct plate


Genotype:
F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-
Reference

Boyer, H. W., Roulland-Dussoix, D. J. Mol. Biol., (1969) 41: 459-472


Competent Cell Type:
Chemically


Transformation Efficiency (cfu/μg):
>1x10
9


Blue-Whiet Screening:
No


Strain:
Hybrid


Reduces Recombination:
Yes


Cloning Methylated DNA:
Yes


Improves Plasmid Quality:
Yes


Preparing Unmethylated DNA:
Yes


T1 Phage Resistant:
No


RecA Deficient:
Yes