商品编号


DA-196



品牌


MCLab



公司


MCLAB.Inc



公司分类


Competent Cells



Description

96x50μl (96-tube rack), >1x10?


Size

1 Ea

商品信息

DH5a? is the most frequently used
E. coli
strain for routine cloning applications.




Description:
DH5a? is the most frequently used
E. coli
strain for routine cloning applications. In addition to supporting blue/white screening recA1 and endA1 mutations in DH5a? increase insert st
ABI
lity and improve the quality of plasmid DNA prepared from minipreps.
Application:

Highest transformation efficiency

General cloning

Blue-white selection

Plasmid isolation


Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.
Genotype:
F- 80dlacZ M15 (lacZYA-argF) U169 recA1 endA1hsdR17(rk-, mk+) phoAsupE44 -thi-1 gyrA96 relA1
Reference:
Woodcock, D.M., Crowther, D.M., Doherty, J., Jefferson,S., DeCruz, E., Noyer-Weidner, M., Smith, S.S., Michael, M.Z., and Graham, M.W., Nucl. Acids Res. (1989) 17, 3469-3478
Competent Cell Type:
Chemically


Transformation Efficiency (cfu/μg):
>1x10
9


Blue-Whiet Screening:
Yes


Strain:
K12


Reduces Recombination:
Yes


Cloning Methylated DNA:
No


Improves Plasmid Quality:
Yes


Preparing Unmethylated DNA:
Not suitable


T1 Phage Resistant:
No


RecA Deficient:
Yes