商品编号


DH10-100



品牌


MCLab



公司


MCLAB.Inc



公司分类


Competent Cells



Description

10x100μl (10 tubes), >1x10?


Size

1 Ea

商品信息

The DH10B competent
E. coli
has a transformation efficiency of 1 x 10
9
cfu/?g for supercoiled DNA and is ideal for high-efficiency cloning and plasmid propagation. It allows stable replication of high-copy number plasmids. In comparison with DH5a, methylated DNA from genomic preparation?could be efficiently transformed into DH10B, therefor, the DH10B cells are more applicable than DH5a in generating genomic libraries containing methylated cytosine or adenine residues.




Description:
The DH10B competent
E. coli
has a transformation efficiency of 1 x 10
9
cfu/?g for supercoiled DNA and is ideal for high-efficiency cloning and plasmid propagation. It enables stable replications of high-copy number plasmids. In comparison with DH5a, methylated DNA from genomic preparation?could be efficiently transformed into DH10B, therefor DH10B cells are more applicable than DH5a in generating genomic libraries containing methylated cytosine or adenine residues.


Application:

Highest transformation efficiency

General cloning

Blue-white selection

Reduced recombination of cloned DNA (recA1)


Recommended storage condition:
This product should be stored at -80°C. Thaw on ice only before use. Do not re-freeze.


Genotype:
F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 endA1 recA1 deoR Δ(ara,leu)7697 araD139 galU galK nupG rpsL λ--


?
Competent Cell Type:
Chemically


Transformation Efficiency (cfu/μg):
>1x10
9


Blue-Whiet Screening:
Yes


Strain:
K12


Reduces Recombination:
Yes


Cloning Methylated DNA:
Yes


Improves Plasmid Quality:
Yes


Preparing Unmethylated DNA:
Not suitable


T1 Phage Resistant:
No


RecA Deficien:
Yes