商品编号


0234-201M



品牌


Reachbio



公司


Reachbio



公司分类


Human Cord Blood Cells




0.2 million, 0.5 million, 1.0 million


pa_unit-size

0-5-million

商品信息

Product Description

Reachbio

QUALIFIED?

Human Cord Blood CD34
+
Cells

(Mixed Donors)

?





Catalog

Number



Selection

Type



Unit Size

(cells per vial)


Product

Info Sheet




0234-101M

positive

0.2 million

download



0234-201M

positive

0.5 million

download



0234-301M

positive

1.0 million

download




?

Cell Enrichment

Positive Selection
Cryopreserved CD34
+
cells have been isolated from pooled (mixed) human cord blood units using immunomagnetic cell separation techniques.? These cells are positively selected using an antibody that targets the CD34 antigen.
?

Purity
The purity of CD34
+
cells is >90% as measured by flow cytometric analysis.
?

Sample Collection and Processing
Umbilical cord blood (CB) is collected in a 250 mL collection bag containing citrate-phosphate-dextrose (CPD) anticoagulant (25 mL/bag). CB is processed through volume reduction by density centrifugation and CD34
+
cells positively selected from the volume-reduced unit.? CB is processed within 24-30 hours of collection.
?

Pre-Qualified for CFC Assays

These CB CD34
+
cells are
Reachbio

QUALIFIED?
.
? They have been functionally pre-qualified in the colony-forming cell (CFC) assay using
Reachbio
’s ColonyGEL? methylcellulose-based media.? The frequency of myeloid and erythroid
Progen
itors, as well as a recommended plating concentration for the CFC assay, are provided for each batch of these
Reachbio
QUALIFIED?
cells (see Certificate of Analysis).
?

St
ABI
lity and Storage
CD34
+
cells are cryopreserved in CryoStor? CS5, a serum-free freezing medium. Cells are stable for long-term storage at -152
o
C or in the gaseous phase of liquid nitrogen. Storage at -80
o
C is not recommended. ?Once thawed, the cells must be used immediately and have a finite life span in culture.
?

Intended Use
FOR IN VITRO RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO. NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER.
Additional Information



Weight

1 lbs



Tissue Source

Cord Blood




Cell Type

Stem/
Progen
itor Cells




Identifying Surface
Marker

CD34




Donor

Mixed (pooled) donors




Unit Size (million cells/vial)

0.2 million, 0.5 million, 1.0 million




Qualification

Reachbio
QUALIFIED




Format

Cryopreserved