商品编号


0519-101



品牌


Reachbio



公司


Reachbio



公司分类


Human Peripheral Blood Cells




10 million


pa_selection-type

positive

商品信息

Product Description

Human Peripheral Blood CD19
+
B Cells





Catalog

Number



Selection

Type



Unit Size

(cells per vial)


Product

Info Sheet



0519-100

Negative

10 million

download



0519-101

Positive

10 million

download




?

Cell Enrichment

Negative Selection
Negatively selected CD19
+
B Cells are enriched from human peripheral blood (PB) mononuclear cells (MNCs) using an immunomagnetic cell separation method that involves an antibody cocktail that targets and depletes non-CD19
+
cells. ?
Positive Selection
Positively selected CD19
+
B Cells are purified from human peripheral blood (PB) mononuclear cells (MNCs) using an immunomagnetic cell separation method that involves a labeled antibody that targets the CD19 antigen.
?

Sample Collection and Processing
Peripheral blood cells are collected from normal human volunteers via leukapheresis (COBE
?
Spectra MNC collection protocol or equivalent). The mononuclear cell-rich leukocyte layer (leukapheresis product) is continually aspirated by a low flow rate collection pump directly into a collection bag. Immunomagnetic cell enrichment of CD19
+
cells is performed within 30 hours of collection. Donors have been screened and found to be negative for a standard panel of infectious agents that at minimum includes HIV, HBV and HCV. Depending on the specific lot, other infectious agents may also have been tested for (Certificate of Analysis includes lot-specific testing information).
?

Purity
Purity of CD19
+
B Cells is >90% as measured by flow cytometric analysis.
?

St
ABI
lity and Storage
Peripheral blood CD19
+
B Cells are cryopreserved in CryoStor? CS5, a serum-free freezing medium. Cells are stable for long-term storage at -152
o
C or in the gaseous phase of liquid nitrogen. Storage at -80
o
C is not recommended. Once thawed, the cells must be used immediately and have a finite life span in culture.
?

Intended Use
FOR IN VITRO RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO. NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. ?
Additional Information



Weight

1 lbs



Species

Human




Tissue Source

Peripheral Blood




Cell Type

B Cells




Selection Type

Negative, Positive




Identifying Surface
Marker

CD19




Unit Size (million cells/vial)

10 million




Format

Cryopreserved