商品编号


0514-201



品牌


Reachbio



公司


Reachbio



公司分类


Human Peripheral Blood Cells




10 million, 20 million


pa_selection-type

positive


pa_unit-size

20-million

商品信息

Product Description

Human Peripheral Blood CD14
+
Monocytes





Catalog

Number



Selection

Type



Unit Size

(cells per vial)


Product

Info Sheet



0514-100

Negative

10 million

download



0514-101

Positive

10 million

download



0514-201

Positive

20 million

download




?

Cell Enrichment

Negative? Selection
Negatively selected CD14
+
Monocytes are enriched from human peripheral blood (PB) mononuclear cells (MNCs) using an immunomagnetic cell separation method that involves an antibody cocktail that targets and depletes non-CD14
+
cells. ?
Positive Selection
Positively selected CD14
+
Monocytes are purified from human peripheral blood (PB) mononuclear cells (MNCs) using an immunomagnetic cell separation method that involves a labeled antibody that targets the CD14 antigen.
?

Sample Collection and Processing
Peripheral blood cells are collected from normal human volunteers via leukapheresis (COBE
?
Spectra MNC collection protocol or equivalent). The mononuclear cell-rich leukocyte layer (leukapheresis product) is continually aspirated by a low flow rate collection pump directly into a collection bag. Immunomagnetic cell enrichment of CD14
+
cells is performed within 30 hours of collection. Donors have been screened and found to be negative for a standard panel of infectious agents that at minimum includes HIV, HBV and HCV. Depending on the specific lot, other infectious agents may also have been tested for (Certificate of Analysis includes lot-specific testing information).
?

Purity
Purity of CD14
+
Monocytes is >80% (positively selected) and 80% (negatively selected) as measured by flow cytometric analysis.
?

St
ABI
lity and Storage
Peripheral blood CD14
+
monocytes are cryopreserved in CryoStor? CS5, a serum-free freezing medium. Cells are stable for long-term storage at -152
o
C or in the gaseous phase of liquid nitrogen. Storage at -80
o
C is not recommended. Once thawed, the cells must be used immediately and have a finite life span in culture.
?

Intended Use
FOR IN VITRO RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR VETERINARY USE IN VIVO. NOT TO BE USED FOR ANY DIAGNOSTIC, THERAPEUTIC OR CLINICAL APPLICATIONS WHATSOEVER. ?
Additional Information



Weight

1 lbs



Dimensions

8 x 10 x 11 in



Species

Human




Tissue Source

Peripheral Blood




Cell Type

Monocytes




Selection Type

Negative, Positive




Identifying Surface
Marker

CD14




Unit Size (million cells/vial)

10 million, 20 million




Format

Cryopreserved